REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-5hmC	Active Motif	Cat#39769
Anti-5mC	Eurogentec	Cat#BI-MECY-0100; RRID: AB_2616058
Anti-BrdU	Abcam	Cat#ab6326; RRID: AB_305426
Anti-Rad51	Santa Cruz	Cat#sc-8349; RRID: AB_2253533
Anti-Rad21 (anti-Scc1)	Millipore	Cat#05-908; RRID: AB_417383
Anti-Xrcc1	Serotec	Cat#AHP832; RRID: AB_2218473
Anti-γH2AX	Abcam	Cat#ab22551; RRID: AB_447150
Alexa Fluor 488 Goat Anti-Mouse IgG (H+L)	Invitrogen	Cat#A-11029
Alexa Fluor 568 Goat Anti-Mouse IgG (H+L)	Invitrogen	Cat#A-11031
Oregon Green 488 Goat Anti-Rabbit IgG (H+L)	Invitrogen	Cat#O-6381
Alexa Fluor 568 Goat Anti- Rabbit IgG (H+L)	Invitrogen	Cat#A-11011
Alexa Fluor 647 Goat Anti-Rat IgG (H+L)	Invitrogen	Cat#A-21247
Chemicals, Peptides, and Recombinant Proteins
3-Isobutyl-1-methylxanthine (IBMX)	Sigma-Aldrich	Cat#I7018
AZD7762	Sigma-Aldrich	Cat#SML0350
PMS (pregnant mare’s serum)	Intervet Austria	Folligon 1000 I.E.
Caffeine	Sigma-Aldrich	Cat#C8960
Cytochalasin B	Sigma-Aldrich	Cat#C2743
Dimethyloxalylglycine (DMOG)	Sigma-Aldrich	Cat#D3695
Fetal Bovine Serum (FBS)	GIBCO	Cat#10270106
Fetuin	Sigma-Aldrich	Cat#F6131
Goat serum (normal)	Vector Labs	Cat#VECS-1000
hCG (human chorionic gonadotropin)	Intervet Austria	Chorulon 1500 I.E.
Hyaluronidase	Sigma-Aldrich	Cat#H3506
Hydroxyurea	Sigma-Aldrich	Cat#H8627
KU-55933	Sigma-Aldrich	Cat#SML1109
M16 media	Home-made	N/A
M2 media	Home-made	N/A
Mineral oil	Sigma-Aldrich	Cat#M8410
NSC109555	Sigma-Aldrich	Cat#SML0781
NU7026	Sigma-Aldrich	Cat#N1537
PBS, pH7.2 (Ca/Mg free)	Invitrogen	Cat#20012019
PF-477736	Sigma-Aldrich	Cat#PZ0186
Research Vitro Fert media	Cook Austria GmbH	Cat#K-RVFE-50
Research Vitro Wash media	Cook Austria GmbH	Cat#K-RVVWA-50
Tyrode’s solution, acidic	Sigma-Aldrich	Cat#T1788
VE-821	Sigma-Aldrich	Cat#SML1415
Vectashield with DAPI	Vector Labs	Cat#VECH-1200
Critical Commercial Assays
Click-iT EdU Alexa Fluor 647 imaging kit	Invitrogen	Cat#C10340
mMessage mMachine T3 kit	Ambion	Cat#AM1348
Experimental Models: Organisms/Strains
Mouse: B6/129SV	Tachibana-Konwalski, K., IMBA, Vienna, Austria, (Tachibana-Konwalski et al., 2010)	N/A
Mouse: B6/CBAF1	IMBA, Vienna, Austria	N/A
Mouse: Tg(Zp3-Cre)3Mrt/J	The Jackson Laboratory, (Lewandoski et al., 1997)	JAX:003394
Mouse: Scc1fl/fl	Tachibana-Konwalski, K., IMBA, Vienna, Austria, (Seitan et al., 2011)	N/A
Mouse: Scc1fl/flTg(Zp3-Cre)3Mrt/J	This paper	N/A
Mouse: Xrcc1tm1Pmc (Xrcc1fl/fl)	McKinnon, P.J., Dpt. of Genetics, St Jude Children’s Research Hospital, Memphis, TN 38105, (Lee et al., 2009)	N/A
Mouse: Xrcc1tm1Pmc (Xrcc1fl/fl) Tg(Zp3-Cre)3Mrt/J	This paper	N/A
Software and Algorithms
GraphPad Prism	GraphPad Software Inc	http://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://imagej.nih.gov/ij/
Other
Grace Bio-Labs SecureSeal imaging spacer	Sigma-Aldrich	Cat#GBL654002
Thermo Scientific Nunc Lab-Tek Chambered Coverglass	Fisher Scientific	Cat#10778091