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. 2004 Sep 15;114(6):857–866. doi: 10.1172/JCI20014

Figure 3.

Figure 3

Cot/Tpl2 is not required for CpG-DNA–induced ERK phosphorylation. (A) WT and Cot/Tpl2–/– macrophages were unstimulated or stimulated with 1 μg/ml LPS, 1 μg/ml synthetic lipopeptide, or 1 μM CpG-DNA for 30 minutes. Western blot analyses were performed with Ab’s against phospho–ERK1/2, ERK1/2, phospho–p38 MAPK, or p38 MAPK. (B) In vitro kinase assay of anti-JNK1 immunoprecipitates was performed on GST–c-Jun as the substrate. As a control, Western blot analysis was performed with anti-JNK1 Ab. (C) Cot/Tpl2–/– macrophages were pretreated with U0126 or staurosporine for 30 minutes, and then left unstimulated or stimulated with 1 μM CpG-DNA. Western blot analyses were performed with the Ab against phospho–ERK1/2 or ERK1/2.