Figure 4.

Effects of TNF neutralization on T cell–mediated graft dysfunction in vivo. Transplanted human arterial segments were recovered from mice injected with saline alone (open squares) or PBMCs together with anti-TNF mAb (filled triangles) or control mAb (IgG₁) (open triangles) for 2 weeks (n = 4 matched triplicates from two experiments). (A–C) Concentration-dependent responses to PGF_2α (A), nitroprusside (B), and bradykinin (C). *P < 0.05; **P < 0.01; ^#P < 0.001 vs. PBMC + IgG₁ group; n = 3–4. (D) Immunohistochemistry of graft sections stained for human CD3 and HLA-DR. Original magnification: CD3 panels, ×200; HLA-DR panels, ×400. (E and F) RNA transcript levels of iNOS (E) and eNOS (F) in whole tissue sections analyzed by quantitative RT-PCR.