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Contact dependence of EC response to T cells. Epifluorescence of eNOS immunostaining (green) with nuclei costaining (blue) of HUVECs that were pretreated with IFN-γ for 3 days prior to coculture with CD4+ T cells separated by a transwell. (A) ECs and T cells were cocultured in the absence of contact. (B) ECs below a transwell containing T cells in contact with IFN-γ–pretreated ECs. (C) ECs below a transwell containing T cells in contact with untreated (resting) ECs.
