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Immunostaining of pancreas sections for islet cell markers. (A) Representative islets from pancreas sections stained with immunofluorescent antibodies for insulin (upper panels, green), glucagon (middle panels, green), synaptophysin (middle panels, red), and Glut2 (lower panels, red) as described in Methods. (B) Representative islets from pancreas sections from the TKO group, costained for insulin (purple), glucagon (green), and somatostatin (red). Four different TKO islets are shown.
