Figure 10.
Effect of plant sterols on LXR-luciferase reporter activity in CHO-7 cells. CHO-7 cells were plated on day 0 at a density of 2 × 105 cells in 6-well plates. On day one, cells were cotransfected with pCMX/Gal4-LXR-α (0.26 μg/well) and pMH100X4-TK-luc (0.88 μg/well) and pCMX-βGal (0.5 μg/well). Cells were incubated in growth medium containing 50 μΜ compactin and 5 μM mevalonate as described in Methods. After 16 hours the luciferase activities were assayed as described in Methods. The experiment was performed 3 times and the values shown are the results of a representative experiment.