TABLE 3.
Mutant clone | Mean FC for LPV compared to WT ± SDa (% decrease in FC of the mutant upon cotransfection with WTb) at indicated % of WT DNA cotransfected
|
|||
---|---|---|---|---|
0% | 9% | 33% | 50% | |
5 | 25.8 ± 4.1 | 13.5 ± 1.3 (48) | 7.2 ± 1.2 (72) | 6.7 ± 0.9 (74) |
6 | 21.2 ± 7.2 | 7.9 ± 1.2 (63) | 4.5 ± 1.0 (79) | 2.8 ± 0.6 (97) |
7 | 66.2 ± 21.1 | 29.6 ± 10.4 (55) | 6.1 ± 1.2 (91) | 3.0 ± 2.1 (95) |
8 | 276.9 ± 27.4 | 140.9 ± 20.8 (49) | 29.5 ± 6.6 (89) | 9.0 ± 2.4 (97) |
9 | 35.8 ± 11.8 | 21.1 ± 0.36 (41) | 7.2 ± 0.7 (80) | 3.1 ± 0.9 (91) |
10 | 54.5 ± 6.6 | 24.4 ± 3.8 (55) | 5.0 ± 1.7 (91) | 3.0 ± 0.4 (95) |
11 | 150.1 ± 17.1 | 132.6 ± 37.0 (11) | 31.3 ± 2.8 (79) | 16.7 ± 3.5 (89) |
Values were determined from the formula IC50 of mutant either alone or in mixture/IC50 of WT pNL4- 3-Fluc. The mean changes in IC50 were derived from two or three independent experiments, and each experiment contains triplicates.
Values are calculated from the formula (FC of mutant in mixture/FC of mutant alone) × 100.