TABLE 2.
Capture probes directed against amino acid positions 83 and 87 of E. coli GyrA, with consideration of silent mutations at amino acid positions 85 and 89
| Name | Position | Silent mutation position (codon) | Sequence (3′ → 5′)a | Amino acid |
|---|---|---|---|---|
| 83A1(Stop) | 83 | 85 (GTC) | AT GGT GAC TAG GCG GTC TA | Stop codon |
| 83T1(Leu) | 83 | 85 (GTC) | AT GGT GAC TTG GCG GTC TA | Leu |
| 83G1(Trp) | 83 | 85 (GTC) | AT GGT GAC TGG GCG GTC TA | Trp |
| 83C1(Ser) | 83 | 85 (GTC) | AT GGT GAC TCG GCG GTC TA | Ser |
| 83A2(Stop) | 83 | 85 (GTT) | AT GGT GAC TAG GCG GTT TA | Stop codon |
| 83T2(Leu) | 83 | 85 (GTT) | AT GGT GAC TTG GCG GTT TA | Leu |
| 83G2(Trp) | 83 | 85 (GTT) | AT GGT GAC TGG GCG GTT TA | Trp |
| 83C2(Ser) | 83 | 85 (GTT) | AT GGT GAC TCG GCG GTT TA | Ser |
| 83AU(Stop) | 83 | 85 (GTI) | AT GGT GAC TAG GCG GTI TA | Stop codon |
| 83TU(Leu) | 83 | 85 (GTI) | AT GGT GAC TTG GCG GTI TA | Leu |
| 83GU(Trp) | 83 | 85 (GTI) | AT GGT GAC TGG GCG GTI TA | Trp |
| 83CU(Ser) | 83 | 85 (GTI) | AT GGT GAC TCG GCG GTI TA | Ser |
| 87A1(Asn) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT AAC ACG ATT G | Asn |
| 87T1(Tyr) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT TAC ACG ATT G | Tyr |
| 87G1(Asp) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT GAC ACG ATT G | Asp |
| 87C1(His) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT CAC ACG ATT G | His |
| 87A2(Asn) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT AAC ACG ATT G | Asn |
| 87T2(Tyr) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT TAC ACG ATT G | Tyr |
| 87G2(Asp) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT GAC ACG ATT G | Asp |
| 87C2(His) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT CAC ACG ATT G | His |
| 87A3(Asn) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT AAC ACG ATC G | Asn |
| 87T3(Tyr) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT TAC ACG ATC G | Tyr |
| 87G3(Asp) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT GAC ACG ATC G | Asp |
| 87C3(His) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT CAC ACG ATC G | His |
| 87A4(Asn) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT AAC ACG ATC G | Asn |
| 87T4(Tyr) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT TAC ACG ATC G | Tyr |
| 87G4(Asp) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT GAC ACG ATC G | Asp |
| 87C4(His) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT CAC ACG ATC G | His |
| 87AU1(Asn) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT AAC ACG ATI G | Asn |
| 87TU1(Tyr) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT TAC ACG ATI G | Tyr |
| 87GU1(Asp) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT GAC ACG ATI G | Asp |
| 87CU1(His) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT CAC ACG ATI G | His |
| 87A5(Asp) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT GAC ACG ATT G | Asp |
| 87T5(Val) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT GTC ACG ATT G | Val |
| 87G5(Gly) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT GGC ACG ATT G | Gly |
| 87C5(Ala) | 87 | 85 (GTC)/89 (ATT) | GCG GTC TAT GCC ACG ATT G | Ala |
| 87A6(Asp) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT GAC ACG ATT G | Asp |
| 87T6(Val) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT GTC ACG ATT G | Val |
| 87G6(Gly) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT GGC ACG ATT G | Gly |
| 87C6(Ala) | 87 | 85 (GTT)/89 (ATT) | GCG GTT TAT GCC ACG ATT G | Ala |
| 87A7(Asp) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT GAC ACG ATC G | Asp |
| 87T7(Val) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT GTC ACG ATC G | Val |
| 87G7(Gly) | 87 | 85 (GTC)/89 (ATC) | GCG GTC TAT GGC ACG ATC G | Gly |
| 87C7(Ala) | 87 | 85(GTC)/89 (ATC) | GCG GTC TAT GCC ACG ATC G | Ala |
| 87A8(Asp) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT GAC ACG ATC G | Asp |
| 87T8(Val) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT GTC ACG ATC G | Val |
| 87G8(Gly) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT GGC ACG ATC G | Gly |
| 87C8(Ala) | 87 | 85 (GTC)/89 (ATT) | GCG GTT TAT GCC ACG ATC G | Ala |
| 87AU2(Asp) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT GAC ACG ATI G | Asp |
| 87TU2(Val) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT GTC ACG ATI G | Val |
| 87GU2(Gly) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT GGC ACG ATI G | Gly |
| 87CU2(Ala) | 87 | 85 (GTI)/89 (ATI) | GCG GTI TAT GCC ACG ATI G | Ala |
a
Boldface indicates missense mutations; underlining indicates silent mutations.