TABLE 4.
Ribotyping, GeneChip, and PFGE genotyping results
| Strain | Genotyping resulta
|
||
|---|---|---|---|
| GeneChip | Ribotype | PFGE | |
| CDC 1 | 1.1 | XII | USA300 (0.0114) |
| CDC 3 | 1.1 | XII | USA300 (0.0114) |
| CDC 4 | 1.1 | XII | USA300 (0.0114) |
| CDC 6 | 1.1 | XII | USA300 (0.0114) |
| CDC 5 | 1.1 | XII | USA300 (0.0114) |
| CDC 2 | 1.2 | XII | USA300 (0.0114) |
| CDC 19 | 1.3 | XII | USA500 TYPE (0.0004) |
| NCTC 8325 | 1.4 | XIII | ND |
| COL (lab 1) | 1.5 | IX | ND |
| COL (lab 2) | 1.5 | ND | ND |
| COL (repository 1) | 1.5 | ND | ND |
| COL (lab 3) | 1.5 | ND | ND |
| CDC 10 | 2.1 | XI | USA400 (0.0051) |
| CDC 13 | 2.1 | XI | USA400 (0.0051) |
| CDC 12 | 2.2 | XI | USA400 (0.0051) |
| CDC 9 | 2.2 | XI | USA400 (0.0051) |
| MW2 | 2.3 | XI | ND |
| CDC 7 | 2.4 | IV | USA400 (0.0199) |
| CDC 8 | 2.5 | XI | USA400 (0.0051) |
| CDC 14 | 2.6 | X | USA400 (0.0172) |
| MSSA476 | 2.7 | XI | ND |
| CDC 11 | 2.8 | XI | USA400 (0.0080) |
| CDC 21 | 2.9 | VI | USA700 TYPE (0.0097) |
| CDC 16 | 3.1 | V | USA100/800 |
| N315 | 3.2 | ND | ND |
| COL (repository 2) | 3.3 | ND | ND |
| CDC 20 | 3.4 | II | USA600 TYPE |
| CDC 17 | 3.5 | VII | USA100-B (0.0022) |
| Mu50 (1) | 3.6 | ND | ND |
| Mu50 (2) | 3.6 | ND | ND |
| CDC 15 | 4.1 | III | USA600 (0.0121) |
| CDC 18 | 4.2 | VIII | USA200 TYPE |
| MRSA252 | 4.3 | I | ND |
Ribotyping, GeneChip, and PFGE results are shown for each strain. Strains were observed to fit into four major clusters (1 to 4) by GeneChip analysis (Fig. 3A). Individual strains within each of these clusters were further distinguished. For example, GeneChip profiles 2.2 and 2.3 are different strains within cluster number 2. Strains with the same profile numbers are identical. Ribotyping results distinguished strains as belonging to one of 12 different ribogroups (I to XII). PFGE results demonstrated that strains belonged to eight different groups (USA100 to USA800; 80% identity cutoff). Numbers in parentheses represent the strain's identification number. Strains with the same identification number are considered identical. ND, not determined.