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. 2004 Sep;42(9):4137–4140. doi: 10.1128/JCM.42.9.4137-4140.2004

TABLE 2.

API 20 NE profile of the 51 strains of biotype 3a

Substrate % (No.) of positive biotype 3 strains Result with control strain
Expected resultc (% positive)
Biotype 1 Biotype 2
Potassium nitrate 100 (51) + + 100
Tryptophan 100 (51) + + 95
Glucose 0 (0) + + 95
Arginine 0 (0) 0
Urea 0 (0) 1
Esculin 0 (0) 95
Gelatin 100 (51) + + 99
PNPG 0 (0) + + 99
Glucose 73 (37) 9
Arabinose 0 (0) 0
Mannose 61 (31) 10
Mannitol 0 (0) + 9
N-Acetyl-glucosamine 6 (3) 1
Maltose 100 (51) + 6
Gluconate 61 (31) + 28
Caprate 0 (0) 0
Adipate 0 (0) 0
Malate 89 (45) + + 95
Citrate 33 (17) + 91
Phenyl-acetate 0 (0) 0
Oxidaseb 100 (51) + + 100
a

Substrates that give a positive result with all 51 strains are boldfaced. PNPG, p-nitrophenyl-β-d-glucoside.

b

External test.

c

Expected result for V. vulnificus according to the insert chart provided by the manufacturer.