Skip to main content
. 2004 Sep;42(9):4261–4267. doi: 10.1128/JCM.42.9.4261-4267.2004

FIG. 1.

FIG. 1.

Schematic diagram of subtype-specific nested multiplex PCR. The upper part of the figure is the gag gene region diagram of subtypes B, C, CRF01-AE, CRF07-BC, and CRF08-BC (10). The lower part of the diagram shows the primer sets in nested multiplex PCR. Primer pair GagF2/Gage2 are universal primers for HIV-1 M group strains. Primer sets cn-gag-B1/cn-gag-b2, cn-gagC1/cn-gag-c3, cn-gag-E2/cn-gag-e3 are subtype-specific primers for subtypes B, C, and CRF01-AE, respectively. Primer set cn-gag-C1/cn-gag-BC is specific for CRF07- and CRF08-BC.