TABLE 2.

Primer sets for PCR and reaction conditions

Primer set(s)a	Reaction conditions	No. of cycles
GagF2/Gage2	94°C for 5 min, 52°C for 1 min, 72 °C for 2 min	1
	94°C for 30 s, 52°C for 30 s, 72°C for 1 min 30 s	35
	72°C for 10 min	1
306/cn-gag	94°C for 2 min, 50°C for 1 min, 72°C for 2 min	1
	94°C for 30 s, 52°C for 30 s, 72°C for 1 min	35
	72°C for 10 min	1
cn-gag-B1/cn-gag-b2, cn-gag-C1/cn-gag-c3, cn-gag-E2/cn-gag-e3, and cn-gag-C1/cn-gag-BCb	94°C for 2 min, 58°C for 1 min, 72°C for 2 min	1
	94°C for 30 s, 58°C for 30 s, 72°C for 1 min	3
	94°C for 30 s, 57°C for 30 s, 72°C for 1 min	3
	94°C for 30 s, 56°C for 30 s, 72°C for 1 min	3
	94°C for 30 s, 55°C for 30 s, 72°C for 1 min	3
	94°C for 30 s, 54°C for 30 s, 72°C for 1 min	25
	72°C for 10 min	1

^aA slash mark means that the first primer is a forward primer and the second primer is a reverse primer.

^bTouchdown PCR is described for these four primer sets.