TABLE 4.
Comparison of phylogenetic analyses and nested multiplex PCR
| Subtype by phylogenetic analysis | Subtype by nested multiplex PCRa
|
||||||
|---|---|---|---|---|---|---|---|
| B
|
C
|
CRF01-AE
|
Total no. | ||||
| No. positive | No. negative | No. positive | No. negative | No. positive | No. negative | ||
| Euro-American B | 10 | 1 | 0 | 0 | 0 | 0 | 11 |
| Thailand B | 59 | 4 | 0 | 0 | 0 | 0 | 63 |
| C | 0 | 0 | 117 | 6 | 0 | 0 | 123 |
| CRF01-AE | 0 | 0 | 0 | 0 | 47 | 3 | 50 |
| A | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
| D | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
| Total | 69 | 5 | 117 | 6 | 47 | 3 | 252 |
a
The percent sensitivity values for subtypes B, C, and CRF01-AE and for the total subtypes are 93.2, 95.1, 94.0, and 94.3%, respectively. The percent specificities were 100% for all for subtypes.