TABLE 2.
Results of culture, electrophoresis, and microarray analysisa
| Sample no. | Culture results
|
Electrophoresis results
|
Microarray results
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CC | CJ | UB | UC | CC | CJ | HIP | UB | UC | CC | CJ | HIP | |
| 1 | − | + | + | + | − | + | + | + | + | − | + | + |
| 2 | − | − | + | − | − | − | − | + | +d | − | +c | +d |
| 3 | − | + | + | + | − | + | + | + | + | − | + | + |
| 4 | − | + | + | + | − | + | + | + | + | − | + | + |
| 5 | − | + | + | + | − | + | + | + | + | +c | + | + |
| 6 | − | + | + | + | − | − | − | + | + | +c | +d | +d |
| 7 | − | − | + | + | − | + | − | + | + | − | +b | +d |
| 8 | − | − | + | + | − | + | − | + | + | − | +b | +d |
| 9 | − | − | + | + | − | + | + | + | + | − | +b | + |
| 10 | − | − | + | + | − | + | + | + | + | − | +b | + |
| 11 | − | − | + | + | − | + | + | + | + | − | +b | + |
| 12 | − | − | + | + | − | + | + | + | + | +c | + | + |
| 13 | − | + | + | + | − | + | + | + | + | − | + | + |
| 14 | − | + | + | + | − | + | + | + | + | − | + | + |
| 15 | − | + | + | + | − | + | + | + | + | − | + | + |
| 16 | − | + | + | + | − | + | + | + | + | − | + | + |
| 17 | − | − | + | + | − | − | − | + | + | +d | − | − |
| 18 | − | − | + | + | − | − | − | + | + | − | − | − |
| 19 | − | − | + | + | − | − | − | + | + | +c | − | − |
| 20 | − | + | + | + | − | + | + | + | + | − | + | + |
| 21 | − | + | + | + | − | + | + | + | + | − | + | + |
| 22 | − | + | + | + | − | + | + | + | + | − | + | + |
| 23 | − | + | + | + | − | + | + | + | + | − | + | + |
| 24 | − | + | + | + | − | + | + | + | + | − | + | + |
| 25 | − | + | + | + | − | + | + | + | + | − | + | + |
| 26 | − | + | + | + | − | + | + | + | + | − | + | + |
| 27 | + | − | + | + | + | + | − | + | + | + | +b | +d |
| 28 | − | + | + | + | − | + | − | + | + | +c | + | +d |
| 29 | − | − | + | + | + | + | − | + | + | + | + | +d |
| 30 | − | − | + | + | − | + | + | + | + | − | + | + |
| 31 | − | − | + | + | − | + | + | + | + | − | + | + |
| 32 | + | − | + | + | − | − | − | + | + | +c | − | − |
| 33 | − | − | + | + | − | − | − | + | + | +d | − | − |
| 34 | − | − | + | + | − | − | − | + | + | +d | − | − |
| 35 | − | + | + | + | − | + | + | + | + | − | + | + |
| 36 | − | + | + | + | − | + | + | + | + | − | + | + |
| 37 | − | + | + | + | − | + | + | + | + | − | + | + |
| 38e | + | − | + | + | − | + | + | + | + | − | + | + |
| 39 | − | + | + | + | − | + | + | + | + | +c | + | + |
| 40 | − | + | + | − | − | − | − | + | +d | − | +d | +d |
| 41 | − | + | + | − | − | − | − | + | +d | − | +d | +d |
| 42 | − | + | + | + | − | + | + | + | + | − | + | + |
| 43 | − | − | + | + | − | − | − | + | + | +d | − | − |
| 44 | − | − | + | + | + | − | − | + | + | + | − | − |
| 45 | − | − | + | + | + | − | − | + | + | + | − | − |
| 46 | − | + | + | + | − | + | + | + | + | − | + | + |
| 47 | − | − | + | + | + | − | − | + | + | + | − | − |
| 48 | − | − | + | + | − | + | − | + | + | − | + | +d |
| 49 | − | − | + | + | − | − | − | + | + | − | +d | +d |
| 50e | + | − | + | + | − | + | + | + | + | − | + | + |
| 51 | − | − | + | + | − | + | + | + | + | − | + | + |
| 52 | − | − | + | + | + | − | − | + | + | +b | − | − |
| 53 | − | + | + | + | − | + | + | + | + | − | + | + |
| 54 | − | + | + | + | − | + | + | + | + | − | + | + |
| 55 | − | + | + | + | − | + | + | + | + | − | + | + |
| 56 | − | + | + | + | − | + | + | + | + | − | + | + |
| 57 | − | + | + | + | − | + | + | + | + | − | + | + |
| 58 | − | − | + | + | − | − | − | + | + | − | − | − |
| 59 | − | − | + | + | − | − | − | + | + | − | − | − |
| 60 | − | + | + | + | − | + | + | + | + | − | + | + |
| 61 | − | + | + | + | − | + | + | + | + | − | + | + |
| 62 | − | + | + | + | − | + | + | + | + | +c | + | + |
| 63 | − | + | + | + | − | + | + | + | + | +c | + | + |
| 64 | − | + | + | + | − | + | + | + | + | − | + | + |
| 65 | − | − | + | + | − | − | − | + | + | − | − | − |
| 66 | × | × | + | + | − | + | + | + | + | − | + | + |
| 67 | × | × | + | + | + | + | + | + | + | + | + | + |
| 67 | × | × | + | + | + | + | + | + | + | + | + | + |
| 68 | × | × | + | + | + | − | − | + | + | + | − | − |
| 69 | × | × | − | − | − | − | − | − | − | − | − | − |
| 70 | × | × | − | − | − | − | − | − | − | − | − | − |
Multiplex PCR was performed with DNA isolated from 65 pooled cloacal fecal samples, 3 samples containing chromosomal reference DNA, and 2 negative control samples. The samples tested by multiplex PCR were analyzed by both capillary electrophoresis and hybridization to microarrays. +, positive signal; −, no detectable signal; ×, not done. The abbreviations in the table match the abbreviations for the primer pairs and capture probes used: UB, universal bacterial; UC, universal Campylobacter; CJ, C. jejuni Cj0046 gene; HIP, C. jejuni hippuricase gene; CC, C. coli.
Positive by microarray analysis and electrophoresis but not by culture.
Positive by microarray analysis but not by electrophoresis or culture.
Positive by microarray analysis but not by electrophoresis.
The positive signal was altered between culture and PCR-based analysis.