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Real-time monitoring of the TRC reactions for the RNA calibrators. (A, C, and E) Fluorescent profile of the TRC reaction for the RNA calibrators (A, tdh RNA; C, trh1 RNA; and E, trh2 RNA); the initial copies of the calibrator mRNA (0, 103, 104, 105, 106, and 107) are indicated. (B, D, and F) Correlation between the initial copies of the mRNA calibrators and their detection times (B, tdh RNA; D, trh1 RNA; F, trh2 RNA).
