TABLE 1.
Detection of tdh, trh1, and trh2 genes and tdh1-, and trh2-specific mRNAs in various V. parahaemolyticus strains
| Strain | Isolation
|
Detectiona of:
|
Detectionb of mRNA specific to:
|
Strain designation in Fig. 4 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sourcec | Locationd | Date (yr) | O:K serovar | tdh | trh1 | trh2 | tdhe | trh1 | trh2 | ||
| AQ3815 | C | IT (Philippines) | 1983 | 4:12 | + | − | − | + (14.1) | − | − | 57 |
| WP1 | C | Japan | Before 1983 | 4:12 | + | − | − | + (15.3) | − | − | 53 |
| VP108 | C | India | 1996 | 3:6 | + | − | − | + (16.4) | − | − | 43 |
| W-3995 | C | Bangladesh | 1981 | 11:46 | + | − | − | + (15.7) | − | − | 48 |
| AQ3810 | C | Singapore | 1983 | 3:6 | + | − | − | + (16.0) | − | − | 44 |
| KX-V138 | C | IT (Indonesia) | 1995 | 3:6 | + | − | − | + (17.4) | − | − | 26 |
| Y-24634 | C | Bangladesh | 1983 | 8:22 | + | − | − | + (17.4) | − | − | 27 |
| AL-37223 | C | Bangladesh | 1996 | 3:6 | + | − | − | + (16.4) | − | − | 36 |
| Q-9604 | C | Bangladesh | 1977 | 4:8 | + | − | − | + (15.7) | − | − | 47 |
| T-26233 | C | Bangladesh | 1979 | 5:15 | + | − | − | + (16.0) | − | − | 45 |
| 97LVP2 | C | Laos | 1997 | 3:6 | + | − | − | + (16.3) | − | − | 37 |
| AN-5034 | C | Bangladesh | 1998 | 4:68 | + | − | − | + (16.5) | − | − | 40 |
| DOH812-14 | C | Taiwan | 1997 | 3:6 | + | − | − | + (16.8) | − | − | 30 |
| BE98-2029 | C | United States | 1998 | 3:6 | + | − | − | + (14.3) | − | − | 25 |
| VP26 | C | Thailand | 1998 | 3:6 | + | − | − | + (16.5) | − | − | 39 |
| VP232 | C | India | 1998 | 4:68 | + | − | − | + (15.7) | − | − | 49 |
| VP1 | C | Korea | 1998 | 3:6 | + | − | − | + (16.6) | − | − | 33 |
| D-1-81 | C | Japan | 1981 | 4:10 | + | − | − | + (16.9) | − | − | 29 |
| D-21-82 | C | Japan | 1982 | 1:38 | + | − | − | + (15.7) | − | − | 50 |
| D-53-83 | C | Japan | 1983 | 1:38 | + | − | − | + (15.8) | − | − | 46 |
| D-7-84 | C | Japan | 1984 | 4:8 | + | − | − | + (14.8) | − | − | 56 |
| D-14-85 | C | Japan | 1985 | 2:3 | + | − | − | + (16.5) | − | − | 42 |
| D-73-86 | C | Japan | 1986 | 3:33 | + | − | − | + (15.2) | − | − | 54 |
| D-19-88 | C | Japan | 1988 | 4:53 | + | − | − | + (13.9) | − | − | 58 |
| D-26-90 | C | Japan | 1990 | 4:63 | + | − | − | + (16.6) | − | − | 35 |
| F192-1 | E (seafood) | Philippine | 1999 | 5:68 | + | − | − | + (16.8) | − | − | 31 |
| D-1-93 | C | Japan | 1993 | 1:56 | + | − | − | + (15.5) | − | − | 51 |
| D-4-94 | C | Japan | 1994 | 4:55 | + | − | − | + (12.4) | − | − | 59 |
| D-3-96 | C | Japan | 1996 | 1:56 | + | − | − | + (15.0) | − | − | 55 |
| TCVP60 | C | Thailand | 1999 | 4:68 | + | − | − | + (16.5) | − | − | 41 |
| TCVP94 | C | Thailand | 1999 | 1:25 | + | − | − | + (17.0) | − | − | 28 |
| TCVP197 | C | Thailand | 1999 | 1:41 | + | − | − | + (16.8) | − | − | 32 |
| 00F390-1 | E (seafood) | Indonesia | 2000 | 12:UT | + | − | − | + (18.4) | − | − | 24 |
| PS02839 | C | Vietnam | 1997 | 3:75 | + | − | − | + (16.6) | − | − | 38 |
| PS06011 | C | Vietnam | 1997 | 4:12 | + | − | − | + (16.6) | − | − | 34 |
| PS14945 | C | Vietnam | 1999 | 11:UT | + | − | − | + (15.4) | − | − | 52 |
| 58 | E | India | 2000 | 7:19 | + | − | − | + (10.5) | − | − | 60 |
| 91A1437 | C | United States | 1991 | 1:UT | + | + | − | + (16.7) | + (14.6) | − | 10 |
| VP104 | C | India | 1996 | 1:UT | + | + | − | + (17.9) | + (16.1) | − | 2 |
| AQ3855 | C | IT (Philippines) | 1983 | 6:18 | + | + | − | + (17.1) | + (15.0) | − | 3 |
| AQ4889 | C | IT (Thailand) | 1993 | 4:12 | + | + | − | + (16.6) | + (16.0) | − | 14 |
| KX-V47 | C | IT (Thailand) | 1995 | 5:UT | + | + | − | + (16.9) | + (14.9) | − | 5 |
| TX-98-792-807-27 | E (oyster) | United States | 1998 | 8:74 | + | + | − | + (19.3) | + (16.2) | − | 1 |
| AQ3776 | C | IT (Thailand) | 1983 | 10:71 | + | + | − | + (12.8) | + (15.0) | − | 22 |
| AQ3860 | C | IT (Philippines) | 1983 | 6:46 | + | + | − | + (16.4) | + (15.1) | − | 13 |
| 94A-7175 | C | United States | 1994 | 4:9 | + | + | − | + (17.0) | + (15.4) | − | 23 |
| 225 | C | Thailand | 1990 | 5:11 | + | + | − | + (15.9) | + (14.6) | − | 18 |
| 91A-3992 | C | United States | 1991 | 1:56 | + | + | − | + (16.9) | + (14.4) | − | 6 |
| 90A-5598 | C | United States | 1990 | 4:63 | + | + | − | + (16.7) | + (16.0) | − | 9 |
| 88A-5320 | C | United States | 1988 | 4:12 | + | + | − | + (16.6) | + (17.4) | − | 11 |
| AQ4781 | C | IT (Hong Kong) | 1992 | 1:UT | + | − | + | + (16.4) | − | + (10.5) | 15 |
| AQ4969 | C | IT (Indonesia) | 1994 | 1:UT | + | − | + | + (16.9) | − | + (12.1) | 7 |
| 275 | C | Thailand | 1990 | 1:1 | + | − | + | + (13.9) | − | + (13.9) | 4 |
| AQ3857 | C | IT (Hong Kong) | 1983 | 1:1 | + | − | + | + (14.7) | − | + (12.0) | 20 |
| AQ4433 | C | IT (Thailand) | 1989 | 1:UT | + | − | + | + (15.4) | − | + (11.7) | 19 |
| AQ4405 | C | IT (Thailand) | 1989 | 3:72 | + | − | + | + (13.5) | − | + (11.8) | 21 |
| AQ4704 | C | IT (Singapore) | 1992 | 1:UT | + | − | + | + (15.8) | − | + (11.7) | 17 |
| AQ4815 | C | IT (Thailand) | 1993 | 1:69 | + | − | + | + (16.9) | − | + (11.0) | 8 |
| AQ4966 | C | IT (Thailand) | 1994 | 1:UT | + | − | + | + (16.3) | − | + (11.9) | 16 |
| KX-V132 | C | IT (Philippines) | 1995 | 3:UT | + | − | + | + (16.6) | − | + (10.9) | 12 |
| 90A-7408 | C | United States | 1990 | 4:12 | − | + | − | − | + (15.8) | − | |
| AQ3749 | C | IT (Singapore) | 1983 | 3:6 | − | + | − | − | + (15.7) | − | |
| AQ4019 | C | IT (Maldives) | 1985 | 3:6 | − | + | − | − | + (16.6) | − | |
| AQ4037 | C | IT (Maldives) | 1985 | 3:6 | − | + | − | − | + (14.2) | − | |
| AQ4235 | C | IT (Thailand) | 1987 | 3:6 | − | + | − | − | + (15.4) | − | |
| AQ4299 | C | IT (Thailand) | 1987 | 3:6 | − | + | − | − | + (15.8) | − | |
| AQ4644 | C | IT (Thailand) | 1991 | 3:6 | − | + | − | − | + (14.8) | − | |
| AQ4733 | C | IT (Singapore) | 1992 | 3:6 | − | + | − | − | + (15.7) | − | |
| AQ4853 | C | IT (Hong Kong) | 1993 | 3:6 | − | + | − | − | + (15.0) | − | |
| AQ4901 | C | IT (Thailand) | 1993 | 3:6 | − | + | − | − | + (15.0) | − | |
| AQ3960 | C | IT (Hong Kong) | 1984 | 4:63 | − | + | − | − | + (15.9) | − | |
| AQ4093 | C | IT (Maldives) | 1986 | 3:6 | − | + | − | − | + (15.6) | − | |
| AQ4095 | C | IT (Maldives) | 1986 | 3:6 | − | + | − | − | + (14.1) | − | |
| AQ4129 | C | IT (Philippines) | 1986 | 3:6 | − | + | − | − | + (15.2) | − | |
| 95A-4675 | C | United States | 1995 | 11:15 | − | − | + | − | − | + (12.4) | |
| AT4 | E (seawater) | Japan | Before 1987 | 4:37 | − | − | + | − | − | + (10.1) | |
| 93A-5463 | C | United States | 1993 | 5:UT | − | − | + | − | − | + (10.8) | |
| 91A-5992 | C | United States | 1991 | 3:59 | − | − | + | − | − | + (9.8) | |
| VP18 | C | India | 1994 | 1:UT | − | − | + | − | − | + (11.2) | |
| VP41 | C | India | 1995 | 1:33 | − | − | + | − | − | + (11.1) | |
| X-13261 | C | Bangladesh | 1982 | 4:UT | − | − | + | − | − | + (11.1) | |
| W-7529 | C | Bangladesh | 1981 | 13:UT | − | − | + | − | − | + (10.8) | |
| W-8274 | C | Bangladesh | 1981 | 1:UT | − | − | + | − | − | + (9.4) | |
| AB-8950 | C | Bangladesh | 1986 | 1:25 | − | − | + | − | − | + (10.3) | |
| Q-6030 | C | Bangladesh | 1977 | 5:UT | − | − | + | − | − | + (10.2) | |
| Q-14337 | C | Bangladesh | 1977 | 3:UT | − | − | + | − | − | + (12.0) | |
| U-5880 | C | Bangladesh | 1980 | 1:56 | − | − | + | − | − | + (10.8) | |
| U-12122 | C | Bangladesh | 1980 | 1:UT | − | − | + | − | − | + (12.4) | |
| U-11868 | C | Bangladesh | 1980 | 4:11 | − | − | + | − | − | + (10.2) | |
| AQ4135 | C | IT (Thailand) | 1986 | 1:UT | − | − | + | − | − | + (10.4) | |
| BAC-98-3574 | C | United States | 1998 | 4:55 | − | − | + | − | − | + (9.8) | |
| VP230 | C | India | 1998 | 1:UT | − | − | + | − | − | + (11.5) | |
| VP276 | C | India | 1998 | 2:28 | − | − | + | − | − | + (10.0) | |
| 68/5 | E (seafood) | Thailand | 1998 | 11:36 | − | − | + | − | − | + (10.0) | |
| AN-20865 | C | Bangladesh | 1998 | 1:UT | − | − | + | − | − | + (15.0) | |
| VP6 | C | India | 1994 | 1:UT | − | − | + | − | − | + (10.7) | |
| DOH272 | C | Taiwan | 1996 | 3:6 | − | − | − | − | − | − | |
| VP56 | E | Japan | 1983 | 2:28 | − | − | − | − | − | − | |
| KV9 | E | Korea | 1998 | 5:17 | − | − | − | − | − | − | |
| 4060 | E | Thailand | 1998 | 3:12 | − | − | − | − | − | − | |
| F25-1 | E (seafood) | Malaysia | 2000 | 5:UT | − | − | − | − | − | − | |
| APCC VP00017 | E | Japan | 2000 | 3:6 | − | − | − | − | − | − | |
| PS01972 | C | Vietnam | 1997 | 8:39 | − | − | − | − | − | − | |
b
Examined by TRC methods.
c
C, clinical; E, environmental. More detailed information for the environmental strain is indicated in the parenthesis if available.
d
IT, international travelers. The origin of travel is indicated in parentheses.
e
The detection time in minutes (the time to reach the cutoff value after initiation of the reaction) is indicated in parentheses.