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. 2004 Sep;42(9):4284–4292. doi: 10.1128/JCM.42.9.4284-4292.2004

TABLE 2.

Oligonucleotide primers and probes used in TRC assays for the trh1- and trh2-specific mRNAs

Target mRNA, primer, and probe Positiona Sequence (5′-3′)b
trh1
    Promoter primer 429-453 AATTCTAATACGACTCACTATAGGGAGATATTCTTCTGTTAGTGATTTCGTTG
    Antisense primer 562-582 ATGATGATTTATTGGAAATAC
    Scissor probe 414-433 GAATAGTTCTGATTTAGGCT
    INAF probe 545-561 ACATAACAAA*CATATGCC
trh2
    Promoter primer 99-121 AATTCTAATACGACTCACTATAGGGAGAAAATCATTCGCGATTGATCTGCCA
    Antisense primer 322-341 GTGACCATTGATGTTGACTG
    Scissor probe 84-103 GATTTAGATATTGAAAATAT
    INAF probe 259-278 CGATTGA*CCGTATACATCTT
a

The numbers correspond to the positions in the nucleotide sequence deposited in GenBank (accession numbers for the trh1 [AB112353] and trh2 [M88112] genes).

b

The underlined sequence is the T7 RNA polymerase-binding sequence. The base linked to oxazole yellow is indicated by the asterisk.