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. 2004 Oct;78(19):10320–10327. doi: 10.1128/JVI.78.19.10320-10327.2004

FIG. 2.

FIG. 2.

Cytotoxic activities of CD8+ T cells expanded from muscle cultures. (A) Chromium release assay to measure the cytotoxic activity directed to the immunodominant Tax 11-19 epitope of CD8+ T cells sorted from muscle cultures (performed in absence of mitogen), compared to PHA-stimulated CD8+ T cells from blood. For 4 days, PHA-stimulated CD4+ T lymphocytes from blood (in which HTLV-1 antigen production was still undetectable) incubated with or without the immunodominant Tax 11-19 epitope (10 μM) were used as targets cells (effector/target ratio, 40:1). (B) Chromium release assay to detect the ability of CD8+ T cells sorted after culture of biopsy 2 to kill chronically infected cells (CB-CD4/HTLV) at two effector/target ratios. Nonproducing autologous PHA-stimulated CD4+ cells were also used as a negative control. A percentage of cell lysis up to 10% was considered significant.