FIG.2.

Vero cells (A to L and Q to T) and CHO cells (M to P) were infected for 12 h with the Ba71v and Uganda strains of ASFV, respectively, and then incubated with 1 μg of BFA/ml (A to D and M to P), 1 mM DTT (E to H and Q to T), or 50 mM sodium arsenite (Arse) (I to L) for 4 h before fixation with 4% paraformaldehyde. The cells in panels Q to T were incubated throughout the experiment with 50 μg of AraC/ml to inhibit DNA synthesis. Cells were stained with either R30 (anti-pY118L) or biotinylated anti-p30 and anti-CHOP antibodies as well as DAPI dye. Primary antibodies were visualized with appropriate secondary reagents conjugated to Alexa⁴⁸⁸ or Alexa⁵⁹⁴. Digital sections (0.2 μm) were captured at a magnification of ×60 and digitally deconvolved by using Openlab 3.1. Bars, 10 μm. Merged images D, H, L, P and T were created digitally. Images were resized and annotated by using Adobe Photoshop 7.0.