Time course of mRNA expression in wild-type AmEPV as determined by Northern blotting. Each blot contains 3 μg of total RNA per lane. The time points are 3, 6, 9, 12, 15, 18, 24, and 48 h postinfection (hpi). The rightmost three lanes of each blot contain RNA isolated from mock-infected cells at 12, 18, and 24 h, respectively. Each blot was hybridized with a single oligonucleotide to one AmEPV gene. The sequences of the oligonucleotide probes are given in Table 2. (A) AMV255 sod gene. The oligonucleotide probe is near the 5′ end of the gene. (B) AMV139 p4a. (C) AMV147 p4b. (D) AMV054 RAP94. (E) AMV016 thymidine kinase. (F) AMV199 DNA ligase.