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Efficient expression of codon-optimized S protein. A plasmid encoding native S protein or codon-optimized S protein or vector alone was transfected into HEK293T cells and infected or not, as indicated, with recombinant vaccinia virus expressing T7 polymerase. Cells were radiolabeled with [35S]cysteine and -methionine. Lysates were precipitated with ACE2-Ig and analyzed by SDS-PAGE.
