FIG. 5.

HVS ORF73 represses p53 transactivation. (A) We transfected 10 million BJAB cells with the pG13-Luc reporter alone (mock) or in conjunction with LANA, A11 ORF73, C488 ORF73, or p53 expression vectors (as indicated). (B, C, and D) BJAB cells were transfected with the pG13-Luc reporter vector in the absence or presence of (B) LANA, (C) HVS A11 ORF73, or (D) HVS C488 ORF73. Cells were harvested 18 h posttransfection, and luciferase expression was measured as described. Equal transfection efficiencies across samples were confirmed by cotransfection of 3 μg of GFP expression vector as well as by Bradford analysis of total protein content in lysed samples. Expression of HVS ORF73 from strains A11 (panel C, bottom) and C488 (panel D, bottom) and p53 (panels C and D, bottom) was confirmed by electrophoresis of the lysate of 1 million transfected cells and by Western blotting with anti-Myc or anti-p53 antibodies, as indicated.