FIG. 9.

LANA and HVS A11 ORF73 transactivate the E2F promoter but not a mutant E2F promoter. (A) The 3x-wt-E2F-Luc and 3x-mut-E2F-Luc vectors have three wild-type and three mutant copies of the luciferase gene, respectively. (B) We transfected 10 million BJAB cells with 5 μg of 3x-wt-E2F-Luc (light bars) or 3x-mut-E2F-Luc (dark bars) and 0, 5, or 10 μg of E2F, LANA, or HVS A11 ORF73 expression vector. Cells were harvested 18 h later and lysed in reporter buffer (Promega), and luciferase activity was measured as described in Materials and Methods. Transfection efficiencies were measured by cotransfecting 3 μg of GFP expression vector. LANA and A11 expression was confirmed by Western blotting.