FIG. 2.

Sequence analysis of MVMi viruses isolated from SCID mice. (Top) Genetic organization of MVMi. For simplicity, only one of the three NS2 isoforms is shown. Shown are the nucleotide changes with respect to the wt in single viral clones isolated from mouse no. 2, 28, and 29 and sequenced across the nt 253 to 4687 region. The central region of the MVM genome (nt 1730 to 2298) that was sequenced in the viral populations and isolated clones outlined below is shaded. (Bottom) Region in which most selected genetic changes mapped. The figure shows the nucleotide positions of the mutations, triplet codes, and NS2 amino acid changes found in viral clones recovered from the indicated mouse spleens. n, number of clones with identical genotypes in this region. The wt sequence was confirmed with two plaque-isolated clones of the MVMi stock used to inoculate the mice.