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. 2004 Oct;78(19):10738–10746. doi: 10.1128/JVI.78.19.10738-10746.2004

FIG. 2.

FIG. 2.

HPV18- and HPV8 E2-expressing keratinocytes both lose β4-integrin expression, but only HPV18 E2-positive cells display apoptotic nuclei. Primary human keratinocytes were seeded in 24-well plates; transfected with 0.4 μg of pEYFP-C1, pEYFP-HPV8-E2, or pEYFP-HPV18-E2 expression constructs (green fluorescence); fixed; permeabilized after 36 h; and stained with DAPI (blue fluorescence) and anti-β4-integrin primary antibody and Cy3-conjugated secondary antibody (red fluorescence). The overlays are shown in the right panel. Due to low transfection efficiencies of the pEYFP-E2 constructs, only single cells could be recorded at a 400-fold magnification. EYFP-8E2.1 to -3 represent the results from three independent pEYFP-HPV8-E2 transfections. The lower panels represent untransfected cells treated with 1 μM staurosporine for 5 h as a control.