FIG. 6.

HPV8 E2 displaces a cellular factor binding to the BS-II of the β4-integrin promoter. Nuclear extracts prepared from HFKs (HFK-NE) were incubated with ³²P-labeled oligonucleotides containing the BS-III (lane 1) or BS-II (lanes 2 to 9) of the β4-integrin promoter. In the case of BS-II, increasing amounts of baculovirus-expressed histidine-tagged HPV8 E2 protein (His-8E2) (lanes 3, 4, and 5) or His-8E2 elution buffer (lanes 7, 8, and 9) were added, and complexes were analyzed by EMSA. The E2-containing band is indicated by an arrow. The single and double asterisks indicate two different complexes of cellular proteins binding to the BS-II oligonucleotides.