FIG. 7.

(A) Mutations within the E2 BS-II of the β4-integrin promoter abolish HPV8 E2 binding. ³²P-labeled oligonucleotides comprising wild-type or mutated BS-II (BS-II-mut containing the same mutated BS-II as in Fig. 5B) were incubated with 28 μg of nuclear extracts isolated from 293T cells transfected with pEYFP-C1 (lanes 2 and 5) or pEYFP-HPV8-E2 (lanes 3 and 6). Lanes 1 and 4 show the free oligonucleotide controls. (B) Mutations within the E2 BS-II of the β4-integrin promoter do not abolish binding of the E2-displaceable cellular factor (asterisk). Nuclear extracts (10 μg) prepared from HFKs were incubated with ³²P-labeled oligonucleotides containing the BS-II or BS-II-mut and analyzed by EMSA.