TABLE 1.
Virus strain | Cell type | Factory morphologya | % of infected cells with factories that costained with cUb at the following time (h) p.i.b:
|
|||
---|---|---|---|---|---|---|
12 | 24 | 36 | 48 | |||
T3DN | CV-1 | Globular | 90 ± 2 | 90 ± 5 | 91 ± 5 | 88 ± 3 |
HeLa | Globular | NDc | 49 ± 18 | ND | 85 ± 6 | |
L929 | Globular | ND | 85 ± 5 | ND | 83 ± 3 | |
T1L | CV-1 | Filamentous | 6 ± 1 | 8 ± 3 | 9 ± 2 | 8 ± 3 |
HeLa | Filamentous | ND | 2 ± 2 | ND | 14 ± 6 | |
L929 | Filamentous | ND | 4 ± 3 | ND | 3 ± 1 |
For CV-1 cells, in which counts were performed, filamentous indicates that ≥96% of cells displayed obvious filaments, and globular indicates that ≤1% of cells displayed obvious filaments.
Results are means of three determinations ± standard deviations; the MOI was 5 PFU/cell. Factories were identified by staining with anti-μ2 or anti-μNS serum. cUb was identified by staining with MAb FK2.
ND, not determined.