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Effect of temperature on viral factory morphology and costaining for cUb
| Virus strain | Temp (°C) | % of infected CV-1 cells with factories that area:
|
|
|---|---|---|---|
| Filamentous | Costained for cUb | ||
| T3DN | 37 | 2 ± 3 | 90 ± 1 |
| 31 | 32 ± 5 | 71 ± 3 | |
| T1L | 37 | 99 ± 0 | 7 ± 1 |
| 31 | 99 ± 0 | 22 ± 9 | |
Results are means of three or four determinations ± standard deviations; the MOI was 5 PFU/cell. Factories were identified by staining with anti-μ2 serum. cUb was identified by staining with MAb FK2.
