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. 2004 Oct;78(19):10715–10723. doi: 10.1128/JVI.78.19.10715-10723.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 4. — In situ hybridization codetecting TYLCSV and TYLCV in doubly infected L. esculentum (a1 and a2) and N. benthamiana (b1 and b2) tissues, from leaves (a1 and b1) and stems (a2 and b2) 21 to 28 dpi. Infected nuclei are indicated by dark staining precipitated from NBT-BCIP (indicated by arrows). All sections were hybridized with a biotin-labeled mixed probe containing TYLCSV plus TYLCV. Accumulation of both viruses is restricted to nuclei from phloem parenchymal cells and companion cells. X, xylem; Ph, phloem; Par, nonvascular parenchyma. Sections were examined by DIC microscopy. Bars, 50 μm.