FIG. 6.

In situ hybridization of purified nuclei from N. benthamiana and L. esculentum plants agroinoculated with TYLCSV (b1 and c1), TYLCV (b2 and c2), or both (a, b3, and c3). Nuclei were fixed and hybridized simultaneously with a biotin-labeled TYLCSV-specific probe and a DIG-labeled TYLCV-specific probe. Red coloring represents TYLCSV, blue coloring represents TYLCV, and purple coloring represents both viruses. (a) General view of isolated nuclei in N. benthamiana by DIC microscopy. Arrows indicate nuclei infected with TYLCSV (red) or TYLCV (blue). (b and c) Close-up images of isolated nuclei from N. benthamiana (b1, b2, and b3) or L. esculentum (c1, c2, and c3) plants by light microscopy are shown. Bars, 25 μm (a) or 10 μm (b1, b2, b3, c1, c2, and c3).