FIG. 4.

gD expression is not affected by the gE-specific siRNA. HaCaT cells were transfected with 10 nM siRNA and then infected with 25,000 PFU of WT HSV-1. At 17 h postinfection, gD and gE expression on the cell surface or after permeabilization were measured by flow cytometry. (a) Total gE; (b) cell surface gD; (c) total gD. Color key: blue, isotype control; red, sigE; black, WT without siRNA; green (shown in panel b), siCRK. This result is representative of the results from three repetitions.