FIG. 6.

mRNA expression from infected HaCaT cells demonstrates specific suppression of the gE transcript by sigE. siRNA-treated and untreated cells were harvested from 12-well plates 48 h after inoculation with 25 PFU of HSV-1. Total RNA was isolated, separated by polyacrylamide gel electrophoresis, and transferred to a nitrocellulose membrane for Northern blot analysis. WT, WT HSV-1 infection; gEnull, gE-deletion virus; sigE, gE-specific siRNA followed by WT infection; siCRK, negative control siRNA followed by WT infection. The membrane was probed for gE and GAPDH as indicated. This result is representative of the results from three trials.