TABLE 2.
Changes in virus populations during systemic infection of Cucumber mosaic virus
| Plant no. and tissue originb | Expt no. and characteristicsa
|
|||
|---|---|---|---|---|
| 1
|
2
|
|||
| No. of mutants recovered | Compositions of the population | No. of mutants recovered | Compositions of the population | |
| 1, In | 12 | abcdefgijkmn | 12 | abcdefgijkmn |
| 1, A | 4 | eijn | 5 | adejn |
| 1, B | 6 | degijk | 5 | cdjkn |
| 1, C | 6 | degijk | 6 | cdegjn |
| 2, In | 12 | abcdefgijkmn | 12 | abcdefgijkmn |
| 2, A | 6 | cdgikn | 5 | acdin |
| 2, B | 5 | cdgkn | 6 | cdgijn |
| 2, C | 5 | cdikn | 6 | cdfgin |
| 3, In | 12 | abcdefgijkmn | 12 | abcdefgijkmn |
| 3, A | 8 | abcdeijn | 8 | acdeijmn |
| 3, B | 8 | abcdjmnj | 8 | acdeijmn |
| 3, C | 8 | abefgijn | 8 | abdeijmn |
For experiment 1, the population of the mutant viruses was inoculated to a small area of the fifth leaf on tobacco at the seven-leaf stage, and the inoculated leaves were detached at 2 dpi. For experiment 2, the population of mutant viruses was inoculated to the whole fifth leaf of tobacco at the seven-leaf stage, and the inoculated leaves were detached at 6 dpi.
1, 2 and 3 represent the three analyzed plants. In, inoculated leaf; A, B, and C, tissues from different locations of the same systemic leaf (eighth leaf).