TABLE 2.
Relative efficiency of RNA packaging within HCMV particles
| Gene | Ratio of CT values relative to that of UL21.5a
|
|||
|---|---|---|---|---|
| Total particles | NIEP | Infectious virus | DB | |
| UL21.5 | 1.0 | 1.0 | 1.0 | 1.0 |
| T/IRL7 | 1.10 ± 0.06 | NDb | ND | ND |
| UL83(1) | 1.06 ± 0.10 | ND | ND | ND |
| UL83(2) | 1.08 ± 0.10 | 1.08 ± 0.04 | 1.08 ± 0.05 | 1.04 ± 0.03 |
| UL108 | 0.98 ± 0.01 | 0.98 ± 0.02 | 0.96 ± 0.03 | 0.92 ± 0.11 |
| UL110 | 1.02 ± 0.04 | ND | ND | ND |
| UL122 | 1.08 ± 0.07 | ND | ND | ND |
| UL123 | 1.02 ± 0.04 | ND | ND | ND |
| GAPDHc | 1.07 ± 0.02 | ND | ND | ND |
| β-Actin | 1.04 ± 0.02 | 1.04 ± 0.05 | 1.03 ± 0.02 | 1.00 ± 0.02 |
| Cyclin G1 | 1.06 ± 0.02 | ND | ND | ND |
| 21.5YFP | 0.91 ± 0.05d | ND | ND | ND |
| YFP | 1.00 ± 0.04d | ND | ND | ND |
a
1/([CT, virion/CT, cell]/UL21.5).
b
NIEP, noninfectious enveloped particles; DB, dense bodies; ND, not determined.
c
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
d
Normalized to CT ratio for T/IRL7.