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. 2004 Oct;78(19):10636–10649. doi: 10.1128/JVI.78.19.10636-10649.2004

FIG. 5.

FIG. 5.

VSV infection induces IRF-3 phosphorylation in the absence of TLR3 or TRIF. (A) C57/BL TLR3−/− MEFs and wt control MEFs were infected with VSV (MOI of 10) or treated with poly(I) · poly(C) dsRNA for 0 to 6 h. WCE (50 μg) derived from VSV-infected cells were analyzed by SDS-7.5% PAGE; transferred to nitrocellulose; and blotted for IRF-3, TLR3, and actin as indicated. (B) WCE (50 μg) derived from dsRNA-treated TLR3−/− MEFs and wt control MEFs were analyzed as described for panel A. (C) WCE (40 μg) prepared from TRIF−/− or TLR3−/− MEFs were infected with VSV (MOI of 10) for 9 h and immunoblotted for IRF-3 and VSV N as indicated. hpt, hours posttreatment.