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. 2004 Oct;78(19):10449–10459. doi: 10.1128/JVI.78.19.10449-10459.2004

FIG. 1.

FIG. 1.

Structural analysis of the genomes of recombinant viruses. (A) Ethidium bromide-stained agarose gel of BglII-digested DNA isolated from infected cells. Parental BAC virus (Parental), gp150 mutant (vgp150Δ), and revertant (vgp150R) are shown. (B) Southern blot analysis of the gel shown in panel A with a digoxigenin -labeled probe spanning nucleotide positions 68650 to 69407 of MHV-68, recognizing a 5.3-kbp BglII fragment in the parental BAC virus and in the revertant virus, respectively, and a 6.6-kbp BglII fragment in the gp150 mutant virus (indicated by white dots in panel A and by an arrowhead in panel B). Marker sizes (in kilobase pairs) are indicated on the left.