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. 2004 Oct;78(19):10479–10489. doi: 10.1128/JVI.78.19.10479-10489.2004

FIG.3.

FIG.3.

Intracellular localization of JSRV Env proteins. Confocal microscopy of 293T cells transiently transfected with the empty expression vector pCDNA 3.1 (A), the HA-tagged full-length expression plasmid ΔGPHA (B), the HA-tagged SU deletion construct ΔGP SUΔ103-352HA, ΔGP SUΔ103-402HA, ΔGP SUΔ253-302HA, or ΔGP SUΔ353-402HA (C, D, E, and F, respectively), or the HA-tagged SU Env PstI insertion construct ΔGP SU102/103PstIHA (G) is shown. HA-tagged Env proteins were detected by staining with a fluorescein isothiocyanate-conjugated monoclonal antibody to HA. Bar, 2 μm. Magnification, ×600.