TABLE 2.
Transformation of JSRV Env mutant constructs in 208F cells
| JSRV Env protein | No. of transformed foci at wka:
|
No. of colonies picked at wkb:
|
Foci re-formationc | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 4 | 5 | 6 | 7 | −8 | 4 | 7 | ||
| Mock | 0 | 0 | 0 | 1 | 1 | 1 | ND | 1 | 0/1 | |
| pCDNA 3.1 | 0 | 0 | 0 | 0 | 0 | 1 | ND | 1 | 0/1 | |
| ΔGP | 36 ± 3 | 68 ± 5 | 149 ± 13 | TMTC | 3 | ND | 3/3 | |||
| ΔGP SUΔ14-52 | 13 ± 9 | 27 ± 6 | 69 ± 21 | TMTC | 3 | ND | 3/3 | |||
| ΔGP SUΔ103-152 | 0 | 0 | 0 | 1 | 1 | 1 | ND | 1 | 0/1 | |
| ΔGP SUΔ203-252 | 0 | 0 | 0 | 0 | 0 | 2 | ND | 2 | 0/2 | |
| ΔGP SUΔ253-302 | 0 | 0 | 0 | 0 | 1 | 1 | ND | 1 | 0/1 | |
| ΔGP SUΔ353-402 | 0 | 0 | 0 | 1 | 1 | 2 | ND | 1 | 0/1 | |
| ΔGP SU13/14PstI | 0 | 0 | 0 | 0 | 0 | 0 | ND | ND | ND | |
| ΔGP SU52/53PstI | 21 ± 3 | 34 ± 5 | 59 ± 7 | 76 ± 22 | 86 ± 18 | TMTC | 3 | ND | 3/3 | |
| ΔGP SU102/103PstI | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | ND | ND |
| ΔGP SU202/203PstI | 0 | 0 | 0 | 1 | 1 | 1 | ND | 1 | 0/1 | |
| ΔGP Y590F | 0 | 0 | 0 | 1 | 1 | 4 ± 1 | ND | 3 | 0/3 | |
| ΔGP M593T | 0 | 13 ± 6 | 19 ± 5 | 30 ± 3 | 38 ± 1 | 54 ± 4 | 70 ± 5 | 3 | ND | 3/3 |
a
All transfections were performed three times, and the average number of foci at each time is indicated along with the average deviation from the mean. Numbers where no errors are given represent the total foci observed in the three experiments. TMTC, too many to count.
b
Foci were isolated from initial transfectants at either 4 weeks or 7 weeks posttransfection, as indicated. ND, not done.
c
Isolated colonies were reseeded in a Six-well plate and assayed for their ability to re-form foci 2 weeks posttransfer. Results are expressed as number of colonies re-forming transformed foci/number of colonies picked.