Figure 3.

Induction of apoptosis via caspase-3 and activation of PARP. Human melanoma cells were incubated with 20 μg/ml of TP extracts for 6h. Cell lysates were prepared, and subjected to SDS–PAGE. Procaspase-3 and PARP were determined by immunoblotting using specific antibodies. The results are representative of those from three separate experiments.