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. 2004 Sep;1(2):175–185. doi: 10.1093/ecam/neh034

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© 2004, the authors Evidenced-based Complementary and Alternative Medicine, Vol. 1, Issue 2 © Oxford University Press 2004; all rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated.

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HPLC separation of standard authentic compounds. The peaks are:1, propolin A (500 μg/ml); 2, propolin B (500 μg/ml); 3, propolin C (500 μg/ml); 4, propolin D (500 μg/ml); 5, propolin E (500 μg/ml); and 6, propolin F (500 μg/ml). The propolins were analyzed with a reversed-phase preparative HPLC. The conditions were: column-Luna Phenomenex (C18, 250 × 4.6 mm); solvent system, methanol:water (82:18); flow rate: 1.5 ml/min; detection, UV 280 nm.