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Loss of OHC phenotype at 14 weeks of age. Midbasal cochlear sections of WT and BKα–/– animals were triple-stained for prestin (red), KCNQ4 (green), and DAPI (blue) (A); double-stained for synaptophysin (green) and DAPI (blue) (B); and triple-stained for Kir4.1 (red), synaptophysin (green), and DAPI (blue) (C). (D) The vestibular organ (sacculus) was double-stained for KCNQ4 (red) and DAPI (blue). Note the loss of OHC phenotype in BKα–/– mutants. (Bar = 20 μm.)
