Fig. 3.

Evolutionary conservation of essential zebrafish genes. Horizonal lines on the Left represent 315 different genes. The genes are listed in the same order as in Fig. 1. In the first four colored columns, the presence of a colored box indicates the presence of one or more homologous genes (blastp E value of <10^–5) in yeast (either S. cerevisiae or S. pombe), C. elegans, D. melanogaster, or H. sapiens. Green boxes indicate genes without homologues in yeast but with homologues in other SCEs such as Giardia, Plasmodium, Trypanosoma, and/or Chlamydomonas. Thus, yellow boxes represent genes conserved through yeast, green are those found in SCEs other than yeast, red are those found in invertebrates but not SCEs, and blue are vertebrate-specific. The last two genes (with no colored boxes) seem to be fish-specific. The black boxes in the last three columns indicate genes whose mutation leads to one of three phenotypes: cystic kidney, chondrogenesis defects, or reduction or lack of melanocyte pigmentation.