Fig. 3.
Length-yield correlation of purified microperoxidases. (A) Dithionite-reduced minus-air-oxidized difference spectra are shown. The y axis is expanded from 450 to 650 nm. For better resolution, the ΔA of MP370 and MP312 were magnified 3- and 10-fold, respectively. hh. cyt. c, horse heart cytochrome c. (B) Yields of heme peptides after purification over nickel nitrilotriacetic acid calculated from ΔA550–536 nm of the difference spectra. The value of MP86 was set to 100%. (C) Correlation of length and yield of heme peptides. The y axis corresponds to that of B.