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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Curr Opin Microbiol. 2016 Aug 4;34:31–37. doi: 10.1016/j.mib.2016.07.015

Figure 3.

Figure 3

Electron microscopic analysis of Gram-positive bacteria. Fresh bacterial cultures of the firmicutes S. aureus (strain Newman) (A) and the actinobacteria C. diphtheriae (strain NCTC 13129) (B) and A. oris (strain MG1) (C) were deposited onto holey carbon grids, which were rapidly frozen in liquid ethane. Cryo-EM images of the frozen-hydrated specimens were taken on a direct electron detector (Gatan K2) on a cryo-electron microscope (FEI Polara). (D) Shown is an A. oris cell obtained by three-dimensional reconstructions by cryo-electron tomography [56]. Note a less dense layer between the membrane and peptidoglycan; a scale bar of 0.2 μm.