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Sequence and expression of pstk. (A) Nucleotide sequence. The cloned pstk sequence is shown. The ATG initiation and TAA termination codons are indicated in bold. (B) Expression of PSTK in bacteria. Lane 1 shows Coomassie-blue-stained gel, and lane 2 shows Western blotting. PSTK was expressed in E. coli cells as a His-tagged protein; the recombinant protein was isolated and probed with polyclonal antibodies against the amino-terminal peptide of PSTK.
