Figure 4. CaMKII inhibition decreases ROCK activity and MLCK phosphorylation, consistent with increased MLCP and MLCK activity.
(A, B) Representative immunoblots (A) and summary data (B) for p-MYPT and total MYPT (n=4 independent experiments). (C, D) Representative immunoblots (C) and summary data (D) for p-MLCK and total MLCK (n=4 independent experiments). (E, F) Representative immunoblots (E) and summary data (F) for p-MLC20 and total MLC20 (n=5 independent experiments). In all immunoblots, mesenteric arteries of 5–7 Ang-II-treated mice were pooled for one sample. Loading control β-actin. * p<0.05 compared to WT by unpaired, two-tailed t-test.
