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. 2016 Nov 28;198(1):196–204. doi: 10.4049/jimmunol.1600420

FIGURE 2.

FIGURE 2.

Particulates trigger DC-derived IL-2 release independent of the NLRP3 inflammasome. WT, Nlrp3−/−, and Asc−/− DCs were primed with LPS (100 ng/ml) and stimulated with alum (A and C) or MSU (B and D). Levels of secreted IL-2 and IL-1β were measured in supernatants. (E) WT DCs were stimulated with alum in the presence or absence of IL-1RA. (F) Proliferation of CellTrace Violet–labeled OT-II CD4+ T cells adoptively transferred into WT or Nlrp3−/− mice immunized with OVA and alum. Representative graphs of CellTrace Violet dilution and consolidated division indexes in mediastinal lymph nodes are shown. (A–E) Graphs representative of three independent experiments are shown. Error bars represent SD. (F) n = 7 mice from three independent experiments. Error bars represent SEM. Statistical significance was assessed using an unpaired two-tailed t test. ns, not significant.