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. 2016 Dec 4;2016:9130976. doi: 10.1155/2016/9130976

(d) Chelation power of antioxidants

Corresponding method Mechanistic description
Tetramethylmurexide (TMM) assay Free Cu(II) or Zn(II) which is not complexed by phenolics (e.g., tannins) is bound to tetramethylmurexide (TMM). The complexation with TMM is assessed at 482 nm for Cu(II) and at 462 nm for Zn(II) [123].
Ferrozine assay Free Fe(II) that is not complexed by phenolics (e.g., tannins) is bound to ferrozine. The complexation of divalent iron with ferrozine is assessed at 562 nm [123].