Fig. 4.
Penetration depth of synchronous [Ca2+]i oscillations. Islets were treated with 11 mM glucose in one fluid stream of the microfluidic device (shown on left side of bar graph) while treatments were varied in the other stream (shown on right side of bar graph in bold). These treatments included 11 mM glucose (n = 8), 2 mM glucose (n = 11), 2 mM glucose plus 10 μM tolbutamide (Tol) (n = 6), and 2 mM glucose plus 25 μM tolbutamide (n = 9), with n representing the number of individual islets used for each measurement. Also shown on the right side of the bar graph (italicized) is the average glucose concentration observed at the specified distance of penetration, as based on the NAD(P)H response.